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    PCR amplification was performed in an Eppendorf (Merck, Johannesburg, South Africa) thermal cycler starting with 10 min denaturation at 95 ºC, followed by 35 cycles of 30 s denaturation at 94 ºC, 30 s annealing at 58 ºC, 1 min extension at 72 ºC, and a final 10 min extension at 72 ºC.

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    PCR amplification was performed in an Eppendorf (Merck, Johannesburg, South Africa) thermal cycler starting with 10 min denaturation at 95 ºC, followed by 35 cycles of 30 s denaturation at 94 ºC, 30 s annealing at 58 ºC, 1 min extension at 72 ºC, and a final 10 min extension at 72 ºC.

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    All countries in South and Central America have environmental protection legislation that includes requirements for at least some aspects of EIAs. Specifically, in South America the development of EIAs has been hampered by political instability, inefficient bureaucracy, economic stagnation, and external debt (Brito and Verocai 1999).

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